Ambrose is the president & CEO of The Ambrose Group, LLC. She previously worked as vice president of a labor firm, an employment law firm, and a Fortune 500 membership association. Ambrose served as a presidential appointee at the U.S. Department of Labor’s Employment Standards Administration (DOL) from 2002 to 2008. Before her work at the DOL, she was a financial advisor for American Express. Ambrose earned two undergraduate degrees simultaneously, attending both Johns Hopkins University, where she received a B.A. in international relations, and the Peabody Conservatory of Music, where she received a B.M. in vocal performance. She later earned a master of music at Arizona State University. Ambrose was first elected to serve as the national committeewoman for the Republican Party of Maryland in 2012. She previously served as chairwoman of the Maryland Young Republicans and as Secretary of the Young Republicans National Federal (YRNF). The was the Republican Party candidate for election to the U.S. House to represent Maryland's 2nd Congressional District. Ambrose lost in the general election on November 8, 2022.